Throughout its life cycle, each RNA will interact dynamically with numerous RNA-binding proteins (RBPs) that determine how the RNA is processed, where it is localized, whether it is translated and finally how it is degraded. In turn, RBPs can interact with hundreds to thousands of cellular RNAs by recognizing certain sequence motifs or structural elements. Understanding these interactions in a genome-wide manner is crucial for gaining a complete picture of an RBP’s specificity and function.
Individual-nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP) has established itself as a leading method to map the RNA interaction sites of a single RBP-of-interest with high resolution and in a quantitative manner. The major objective of this EMBO Practical Course is to disseminate the knowledge and proficiency in this technically challenging ribonomics method. We will guide the participants through the complete protocol, placing special emphasis on critical optimisation steps and controls. We will further discuss the bioinformatics strategies for analysis of iCLIP data. The hands-on sessions in the laboratory will be complemented by presentations by established scientists that will share their experience on using iCLIP to answer different biological questions.
Speakers & Instructors
To see full programme of the course click HERE.
To register HERE.
Abstract Submission & Abstract deadline 1st March 2018
Chosen Participants Will Be Notified By 9th March 2018
Payment Deadline 29th March 2018
Selected participants will received an email with a link to make the payment no later than 09 March 2018.
The course is limited to 18 participants, which will be selected according to the following criteria:
Only complete applications will be considered. Applications will be evaluated and selected by the organisers based on suitability for the course. Selected participants will be contacted by e-mail by 9 March, 2018. If positions remain open future rounds of selection will be performed, therefore you might be notified later.
For participants that already started with their own iCLIP experiments at home, it will be possible to bring their own samples under certain conditions. If you are interested in this option, please contact firstname.lastname@example.org for more details.
Applicants are required to provide the following information:
The course includes several poster sessions in which participants will explain their research projects. Each participant will be provided with a A0 poster board on which to display the poster.
The poster board surface area is aprox. 90 cm wide and 120 cm high. This is a portrait/vertical format. Materials should be printed on thin poster paper or cardboard, anything heavier will not stay in position. You will be provided with velcro tabs and pins for use in adhering your poster to the board. All participants will present their poster.
A limited number of travel grants are available for eligible participants. Applicants do not need to apply separately for travel grants for this event but should indicate on the registration form if they wish to be considered for a travel grant. Selection of awardees is handled directly by the organizer who will notify all eligible participants. More information is available at EMBO Travel Grants' page.
Participants will be provided accommodation in double shared rooms with other participants in the course (the organizers will prepare the rooming list based on gender).
CRG – Centre for Genomic Regulation c/Dr Aiguader 88